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CellTox Green Cytotoxicity Assay
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CellTox Green Cytotoxicity Assay
CellTox Green Cytotoxicity Assay
CellTox Green Cytotoxicity Assay from Promega
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Citations:
(67)
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CellTox Green Cytotoxicity Assay
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Description
The CellTox Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTox Green Cytotoxicity Assay
Company
Promega
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
G8741
Quantity
10ml
Reactivity
mammal
Sample Type
cells in culture
Sensitivity
<200 cells="">200>
Time
0-72 hours
Molecular Weight
~61kDa
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (67)
(21)
A clinically relevant heterozygous ATR mutation sensitizes colorectal cancer cells to replication stress
Scientific reports
March 31, 2022
Egger T, Bordignon B, Coquelle A.
25-4015-82
, RRID:AB_11150243). The commercial kits used were:
CellTox Green Cytotoxicity assay
(
Promega
,
G8741
),
ViaStain Live caspase 3/7 detection for 2D/3D culture
(
Nexcelom
,
CSK-V0002-1
),
Apoptosis, DNA Damage and Cell Proliferation Kit
(22)
MESH1 knockdown triggers proliferation arrest through TAZ repression
Cell death & disease
March 10, 2022
Sun T, Ding CC, Zhang Y, Zhang Y, Lin CC, Wu J, Setayeshpour Y, Coggins S, Shepard C, Macias E,
et al
.
absorbance at 570 nm. Cell death was measured by the
CellTox Green Cytotoxicity assay
reagents (
Promega
, #
G8741
) (n = 3 biologically independent replicates) at 485/520 nm at 0, 16, 19, 24, 40, 48, 73, and 96 h post
(23)
miR-376a-3p and miR-376b-3p overexpression in Hutchinson-Gilford progeria fibroblasts inhibits cell proliferation and induces premature senescence
iScience
February 18, 2022
Frankel D, Delecourt V, Novoa-Del-Toro EM, Robin JD, Airault C, Bartoli C, Carabalona A, Perrin S, Mazaleyrat K, De Sandre-Giovannoli A,
et al
.
Kit,
Abcam
,
ab126556
FxCycleTM Far Red Stain,
Thermo Fisher Scientific
,
F10348
CellTox™ Green Cytotoxicity Assay
,
Promega
,
G8741
CellTiter-Glo® 2.0
Cell Viability Assay
,
Promega
,
G9241
Autophagy Assay Kit
,Abcam,
ab139484
Pierce™ BCA Protein Assay Kit
(24)
Cell death-inducing cytotoxicity in truncated KCNQ4 variants associated with DFNA2 hearing loss
Disease models & mechanisms
November 1, 2021
Kojima T, Wasano K, Takahashi S, Homma K.
at 37°C and 5% CO2 (33°C in 10% CO2 for HEI-OC1 cells) using a
CellTox Green Cytotoxicity Assay
kit (
G8741
,
Promega
) and
RealTime-Glo Annexin V Apoptosis Assay
kit (
JA1000
,
Promega
) following the manufacturer's
(25)
Comparative epigenetic analysis of tumour initiating cells and syngeneic EPSC-derived neural stem cells in glioblastoma
Nature communications
October 21, 2021
Vinel C, Rosser G, Guglielmi L, Constantinou M, Pomella N, Zhang X, Boot JR, Jones TA, Millner TO, Dumas AA,
et al
.
CellTiter-Glo Luminescent Cell Viability Assay
with
CellTox Green Cytotoxicity Assay
(
Promega
kits
G7570
and
G8741
). Drug treatment on
co-culture
cerebral and intestinal
organoids
Syngeneic GLICO were allowed to develop
(26)
CYP27A1-dependent anti-melanoma activity of limonoid natural products targets mitochondrial metabolism
Cell Chemical Biology
October 1, 2021
Hyelim Cho, Qiong Shen, Lydia H. Zhang, Mikiko Okumura, Akinori Kawakami, Jessi Ambrose, Frederic Sigoillot, Howard R. Miller, Scott Gleim, Amanda Cobos-Correa,
et al.
Assays
CellTiter-Glo® Luminescent Cell Viability Assay
,
Promega
,
G7571
CellTox™ Green Cytotoxicity Assay
,
Promega
,
G8741
"RealTime ready DNA Probes Master, 5 x 1 ml",Roche,Cat#5502381001
Mitochondria Isolation Kit for Cultured Cells
(27)
Metabolic memory underlying minimal residual disease in breast cancer
Molecular systems biology
October 1, 2021
Radic Shechter K, Kafkia E, Zirngibl K, Gawrzak S, Alladin A, Machado D, Lüchtenborg C, Sévin DC, Brügger B, Patil KR,
et al
.
(µM) were tested: 250, 50, 25, 10, 2, 0 (vehicle, water). A
CellTox Green Cytotoxicity Assay
(
Promega
,
G8741
) was performed according to the manufacturer instructions to
measure
cell death after 72 or 48 h of
(28)
Selective activation of PFKL suppresses the phagocytic oxidative burst
Cell
August 19, 2021
Amara N, Cooper MP, Voronkova MA, Webb BA, Lynch EM, Kollman JM, Ma T, Yu K, Lai Z, Sangaraju D,
et al
.
ROCK inhibitor Y-27632
,
Sigma-Aldrich
,
SCM075
Critical commercial
assays
,, CellTox cytotoxicity
assay
,
Promega
,
G8741
"MSD Cytokine
Assay
, 96-well multi-array
Tissue Culture
Kit",
Meso Scale Discovery
,
K151AGB-2
ROS-Glo H2O2 assay
(29)
Inhibitors of VPS34 and fatty-acid metabolism suppress SARS-CoV-2 replication
Cell reports
August 3, 2021
Williams CG, Jureka AS, Silvas JA, Nicolini AM, Chvatal SA, Carlson-Stevermer J, Oki J, Holden K, Basler CF.
assays
,Critical commercial
assays
,Critical commercial
assays
,,
CellTox Green Cytotoxicity Assay
,
Promega
,Cat#
G8741
CytoView-Z 96-well electrode plates,Axion BioSystems,Cat# Z96-IMP-96B ,,
Experimental models
: Cell
(30)
Synergistic efficacy of combined EGFR and HDAC inhibitors overcomes tolerance to EGFR monotherapy in salivary mucoepidermoid carcinoma
Oral Oncology
April 1, 2021
Kshitij Parag-Sharma, Jason Tasoulas, Adele M. Musicant, Carlos H. Viesi Viesi do Nascimento-Filho, Zhichuan Zhu, Chloe Twomey, Pengda Liu, Rogerio M. Castilho, Antonio L. Amelio
post drug addition, the amount of cell death per well was quantified using the CellTox (
Promega
, cat#:
G8741
) kit, according to the manufacturer’s protocol. Briefly, 50 μL of CellTox reagent (diluted
1:200
in
(31)
A Novel Strategy to Study the Invasive Capability of Adherent-Invasive Escherichia coli by Using Human Primary Organoid-Derived Epithelial Monolayers
Frontiers in Immunology
March 29, 2021
Aida Mayorgas, Isabella Dotti, Marta Martínez-Picola, Miriam Esteller, Queralt Bonet-Rossinyol, Elena Ricart, Azucena Salas, Margarita Martínez-Medina
MO, USA. Ref. T8787).Distilled H2O.
CellTox™ Green Cytotoxicity Assay
(
Promega
, Madison, WI, USA. Ref.
G8741
).
Digitonin
(Sigma-Aldrich, Saint Louis, MO, USA., Ref. D141). WC: 100 µg/ml. Equipment Consumables 1.5
(32)
Molecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance
Cell death & disease
February 8, 2021
Bishnu A, Phadte P, Dhadve A, Sakpal A, Rekhi B, Ray P.
Trametinib, and their combination was evaluated by
CellTOX Green® cytotoxicity assay
(
Promega
, WI, USA,
G8741
). Breifly, A2780DualER and OAW42DualER (5000 cells/well) cells were plated overnight in black clear
(33)
Targeted Protein Degradation Phenotypic Studies Using HaloTag CRISPR/Cas9 Endogenous Tagging Coupled with HaloPROTAC3
Current Protocols in Pharmacology
December 17, 2020
Elizabeth A. Caine, Sarah D. Mahan, Rebecca L. Johnson, Amanda N. Nieman, Ngan Lam, Curtis R. Warren, Kristin M. Riching, Marjeta Urh, Danette L. Daniels
viability
assays
, such as CellTiter Glo (
Promega
, cat. no.
G7570
) or CellTox Green (
Promega
, cat. no.
G8741
), will be important to deconvolute protein loss from cell death. In addition, as these are live‐cell
(34)
Colorectal cancer-associated anaerobic bacteria proliferate in tumor spheroids and alter the microenvironment
Scientific Reports
December 1, 2020
Stephen H. Kasper, Carolina Morell-Perez, Thomas P. Wyche, Theodore R. Sana, Linda A. Lieberman, Erik C. Hett
brightfield,
phase contrast
, and
fluorescence
protocols.
Cell lysis
assay
The CellTox™ Green (
Promega
,
G8741
) cytoxicity
assay
was used according to manufacturer’s protocol with slight modification. CellTox™ Green
(35)
Mechanistic Studies of the Multiple Myeloma and Melanoma Cell-Selective Toxicity of the Rpn13-Binding Peptoid KDT-11
Cell chemical biology
November 19, 2020
Dickson P, Simanski S, Ngundu JM, Kodadek T.
was performed. Cell
toxicity assay
Cell death was assessed by Cell ToxGreen reagent (
Promega
Cat#
G8741
). 8,000 cells/well were seeded into 96-well black plates (Grenier) and incubated with compound on 1%
(36)
In vitro function and in situ localization of Multidrug Resistance-associated Protein (MRP)1 (ABCC1) suggest a protective role against methyl mercury-induced oxidative stress in the human placenta
Archives of Toxicology
November 1, 2020
Sebastian Granitzer et al.
RealTime-Glo MT Cell Viability Assay
(
Promega
;
G9711
), while cytotoxicity was measured by
CellTox Green Cytotoxicity Assay
(
Promega
;
G8741
) according to the manufacturer’s protocol. In short, 1 × 103 cells/well were seeded, on the next day
(37)
Biological Properties of a Citral-Enriched Fraction of Citrus limon Essential Oil
Foods
September 14, 2020
Marzia Pucci, Stefania Raimondo, Chiara Zichittella, Vincenza Tinnirello, Valeria Corleone, Gioacchino Aiello, Marta Moschetti, Alice Conigliaro, Simona Fontana, Riccardo Alessandro
Treatment For the detection of cytotoxicity, the
CellTox™ Green Cytotoxicity Assay
(Catalog number
G8741
,
Promega
, Madison, WI, USA) was used. RAW264.7 were cultured in triplicate at 5 × 103 cells per well
(38)
Peucedanum ostruthium
Inhibits E-Selectin and VCAM-1 Expression in Endothelial Cells through Interference with NF-κB Signaling
Biomolecules
August 21, 2020
Lammel C, Zwirchmayr J, Seigner J, Rollinger JM, de Martin R.
concentrations of PO (50, 25, 12.5 µg/mL), and the
CellTox Green Cytotoxicity Assay
(
Promega
, Madison, WI, USA, #
G8741
) was performed according to the manufacturer’s recommendations. Cytotoxicity was judged by morphological
(39)
Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate
Cell
July 23, 2020
Wu CC, Peterson A, Zinshteyn B, Regot S, Green R.
anisomycin for 30 min.
Cell lysis
was
measure
using
CellTox Green Cytotoxicity Assay
kit (
Promega
,
G8741
) following manufacturer’s instructions. 9% Triton X100 was used as a positive control for
cell lysis
. Cell
(40)
Signatures of cell stress and altered bioenergetics in skin fibroblasts from patients with multiple sclerosis
Aging (Albany NY)
July 8, 2020
Jordan M. Wilkins, Oleksandr Gakh, Parijat Kabiraj, Christina B. McCarthy, W. Oliver Tobin, Charles L. Howe, Claudia F. Lucchinetti
using the MTT reduction
assay
or
CellTox Green Cytotoxicity assay
(
Promega
, Madison, WI, USA, Kit #
G8741
). For the
MTT assay
, skin
fibroblasts
were seeded in duplicate at 20,000 cells per well in a 96-well
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CellTox Green Cytotoxicity Assay from Promega
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